ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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ASTM D – Proteins in the latex migrate to the surface and penetrate through the skin.

Some of the proteins can be more harmful than satm. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. While donning and removing powdered gloves, the protein-coated particles become air borne dust and can contaminate large volumes.

ASTM D5712 – 15

Allergen units can range from 10, units per ml for an extract from raw latex film to 1 unit per ml extracted from vinyl glove film negative control or latex with undetectable level of allergenic proteins. For example, someone asmt develop a process for latex protein cleaning, submit the clean latex to ASTM D test and find that ast protein level was reduced from ppm to 20 ppm. Other advantages are an increase in e5712, stability and transport properties.

The fumed silica attaches itself to the rubber particle and substitutes the proteins. Natural proteins are the main reason for Type I latex irritation. D Specification for Rubber Surgical Gloves. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely.

As a consequence of the addition of ammonia for preserving the latex concentrate, the lipid materials are believed to hydrolyze slowly, releasing fatty acids. In most cases the powder is removed by washing with water which further reduces the protein content by extraction. Akron Rubber Development Laboratory, Inc. A previous paper v5712 cleaning latex films with fumed silica5 presented protein results derived by ASTM D Register for a trial account.


It is the responsibility of the user of this aatm to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

Click here to download full list of books. Print 1 2 3 4 ashm page sstarting from page current page. Tables I and II report results with silica at a level of 1 to 5 weight percentage. A definitive procedure that produces a test result: The silica also may serve as a thickening agent.

About 10 percent of medical field employees dealing with dipped asgm products suffer from this allergy. Search book title Enter astmm for book title search. For specific hazard statements, see Section 9. Thompson et al9 that there is a relationship between hydration and compromised barriers in some surgical gloves. A large number estimated in the order asfm magnitude of 10 percent in the U. If you like to setup a quick demo, let us know at support madcad.

Are you sure you want to empty the cart? Summary and conclusions Cost and process advantage of latex dipped with fumed silica Presently, latex goods with relatively low protein content are being manufactured by various complicated methods. Mechanisms of protein cleaning by fumed silica—displacement or attachment The mechanism by which dispersed fumed silica reduces protein content in the latex has not been scientifically established.

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FORMAT Single User Subscription Site License Subscription BOOKS All Books PACKAGES All Packages EDITION to PUBLISHER. Latex protein analysis ASTM D, might not represent a true picture of the latex irritation problem. November 20, Content source: Remember me for one month. There is a major distinction between powdered and powder-free gloves. However, traces still may remain and cause skin reactions. Latex processed without silica has protein residues, see Table IV.


Potential hazards include skin absorption of harmful substances, chemical or thermal burns, electrical dangers, bruises, abrasions, cuts, punctures, fractures and amputations.

However, these methods are good for the coated surface only and proteins migrate to both sides. Protein levels are below 1, the lowest level of detection, as clean as a vinyl film negative control.

Navigation Top Stories Search. Leap latex elisa for antigenic proteins, Volume 61, No.

Removing latex proteins with fumed silica additives | Rubber and Plastics News

This test method covers an analytical test for determining the amount of total d57712 extractable protein associated with NR, latex, and elastomeric products.

After displacement, the protein become mobile and easier to astk off. Washing is minimal and since the latex is clean, there is no need for costly off line post treatments. The following editions for this book are also available ASTM D might be misleading and can cause unfortunate conclusions. Many gloves contain less proteins today, but protein cleaning is an important ongoing issue. It is well known that proteins can transfer from the latex to the powder.

One is to protect the cured latex from sticking to itself during manufacturing process, and the other d57712 to provide internal lubricity while donning the gloves.

According to the literature,8 when the latex is in the tree, the particles are presumed to possess an adsorbed layer of proteins—lipid complex. A general estimate is that only about 20 percent of the total protein evaluated by the ASTM test are harmful. Powder-free latex gloves and other methods of reducing protein content Latex gloves are among the most commonly used critical latex articles.

Glove manufacturers try to avoid powder by producing a powder-free or powder-reduced glove.

Last modified: April 21, 2020