An automated comprehensive isotachophoresis−capillary zone electrophoresis ( ITP−CZE) system is described. The sample is focused in the first capillary by ITP . Instrumentation and fractionation of human serum proteins. An automated free- solution isotachophoresis system (FS-ITP) for preparative fractionation of. An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension.

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We present an inexpensive hand-held device g that implements microchip isotachophoresis ITP with laser induced fluorescence LIF detection. This self-contained instrument integrates the functionality required for high voltage generation onto a microelectronic chip, includes LIF detection and is powered by a universal serial bus USB link connected to a laptop computer. Using this device we demonstrate focusing and detection of a fluorescent species with a limit of detection of pM.

We show that the response of the detector is linear with the initial analyte concentration, making this device suitable for quantitative analysis. We also demonstrate the use of our simulation tools for design and prediction of ITP assays, and validate these results with a demonstration of multiplexed indirect detection of unlabeled analytes performed using the device. We find good agreement between simulations and experimental results.

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Using a label-free isotachaphoresis assay implemented in the hand-held device we detect two explosives and an endocrine disruptor spiked in river waterwith no prior sample processing. The article was received on 10 Maraccepted on 25 May and first published on 23 Jun If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using Copyright Clearance Center.

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Single-electrolyte isotachophoresis using a nanochannel-induced depletion zone.

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Single-electrolyte isotachophoresis using a nanochannel-induced depletion zone.

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Last modified: June 15, 2020